Table I. Rate and extent of FRAP aurora B–GFP or the kinase inactive mutant of aurora B, aurora B(K-R)-GFP.
|
|
Slope
|
t
1/2
|
Recovery
|
n
|
|---|---|---|---|---|
| k | s | % | ||
| Aurora B–GFP | ||||
| Prometaphase centromeres | −0.017 ± 0.007 | 47 ± 24 | 78 ± 13 | 13 |
| Nocodazole centromeres | −0.013 ± 0.005 | 62 ± 19 | 59 ± 12 | 20 |
| Aurora B(K-R)-GFP | ||||
| Prometaphase centromeres | −0.009 ± 0.003 | 84 ± 35 | 48 ± 14 | 13 |
The rate constant k was measured as the negative slope of In(i∞ − it) versus time after photobleaching. Halftime was calculated as t1/2 = ln2*(−1/k), as described in Materials and methods. Values are shown ± SD.