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. 2002 Oct 14;159(1):91–102. doi: 10.1083/jcb.200202114

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Copyright © 2002, The Rockefeller University Press

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Figure 7. — Effect of LPS on neutrophil chemotaxis and Akt phosphorylation induced by IL-8. (a) Neutrophils were pretreated for 1 h with 10 ng/ml LPS and then placed in wells adjacent to optimal concentrations of IL-8 (10.0 pmol). (b) Phosphorylation of Akt in LPS-treated neutrophils stimulated with IL-8 ± the p38 MAPK inhibitor SB203580. Results are shown as mean ± SEM with a minimum n = 9 for chemotaxis assay and n = 3 for Akt phosphorylation. *P < 0.05 compared with IL-8 alone.

Figure 7. — Effect of LPS on neutrophil chemotaxis and Akt phosphorylation induced by IL-8. (a) Neutrophils were pretreated for 1 h with 10 ng/ml LPS and then placed in wells adjacent to optimal concentrations of IL-8 (10.0 pmol). (b) Phosphorylation of Akt in LPS-treated neutrophils stimulated with IL-8 ± the p38 MAPK inhibitor SB203580. Results are shown as mean ± SEM with a minimum n = 9 for chemotaxis assay and n = 3 for Akt phosphorylation. *P < 0.05 compared with IL-8 alone.