Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Oct 14;159(1):69–78. doi: 10.1083/jcb.200206015

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — GAP in the presence of GTP is required for the efficient sorting of the KDEL receptor into reconstituted vesicles, and GTPγS inhibits this process. The two-stage incubation was performed with the first stage using Golgi membrane derived from CHO cells that had been stably transfected with a myc-tagged KDEL receptor. The first stage was performed with either GTP or GTPγS, followed by the second stage either with or without GAP. For the GTPγS conditions, the additional manipulations of pipette shearing and salt treatment were used so that similar levels of COPI vesicles could be generated for comparison. A fraction (10% by volume) of the supernatant derived from the different conditions was applied onto EM grids for immunogold labeling with antibody directed against the myc epitope, followed by quantitation for vesicles with gold particles.

HHS Vulnerability Disclosure