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. 2002 Oct 14;159(1):69–78. doi: 10.1083/jcb.200206015

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Copyright © 2002, The Rockefeller University Press

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Figure 7. — Coated vesicles have stoichiometric levels of COP and GAP, and significantly reduced levels of ARF1. For A, the two-stage incubation was scaled up fivefold with the supernatant derived from the second stage and then loaded at the bottom of a continuous sucrose gradient for equilibrium centrifugation followed by fractionation and immunoblotting with antibodies indicated. Quantitation of β-COP, ARF1, and GAP was performed based on immunoblotting using known amounts of purified proteins, as indicated in B.

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