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Treatment with Brefeldin A abolishes ERK phosphorylation in TGFβ pretreated cells. Immunoblot with a phospho-specific antibody to ERK with protein extracts from CG neurons. Cells were incubated with Brefeldin A (2.5 μM) or vehicle alone for 10 min before treatment with TGFβ or soluble GFRα1 for 3 h, followed by addition of GDNF for 30 min. A quantification of the bands representing phosphorylated ERK normalized to total ERK is shown in the bar diagram.
