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. 2003 Oct 27;163(2):375–384. doi: 10.1083/jcb.200301131

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — Insulin induces tyrosine phosphorylation and association of PLCγ1 with the IR in HepG2 cells and rat hepatocytes. (A) Serum-starved HepG2 cells were pretreated with 200 μM orthovanadate for 30 min before stimulation with 100 nM insulin for 15 min. Anti-PLCγ1^PH immunoprecipitates were blotted with the indicated antibodies. Equal loading was confirmed by reprobing the membranes with anti-PLCγ1^PH. Results shown are representative of three independent observations. (B) A primary culture of rat hepatocytes was treated (or not treated) with 100 nM insulin for 10 min. Lysates were incubated with anti-PLCγ1 antibody or a control mAb (C), and the immunoprecipitates were blotted with anti-IR α-subunit antibody.