Table II. L447 and I449 mutant F protein expression and mAb 21-1 reactivity.
| F1 expressa pGEMb | F1 expressa pCAGGSc | Surface expressd pGEMb | Surface expressd pCAGGSc | 21-1 reactivitye pGEMb | 21-1 reactivitye pCAGGSc | |
|---|---|---|---|---|---|---|
| SV5 F wtf | 100 | 100 | 100 | 100 | 100 | 100 |
| L447G | 61.5 | 70.6 | 85.0 | 61.7 | 77.9 | 56.9 |
| L447A | 103.9 | 120.1 | 107.9 | 92.8 | 86.7 | 75.7 |
| L447V | 114.4 | 96.8 | 107.1 | 99.2 | 82.4 | 73.7 |
| L447I | 113.0 | 90.6 | 103.9 | 110.1 | 99.5 | 99.1 |
| L447F | 15.8 | 9.6 | 10.3 | 15.3 | 135.0 | 104.5 |
| L447W | 65.0 | 75.8 | 51.8 | 79.6 | 194.1 | 123.6 |
| I449G | 22.2 | 19.6 | 9.7 | 8.1 | 4.0 | 30.8 |
| I449A | 46.5 | 49.6 | 72.0 | 63.1 | 76.3 | 74.4 |
| I449V | 117.1 | 119.7 | 97.9 | 92.3 | 86.1 | 79.3 |
| I449L | 86.7 | 105.0 | 83.1 | 86.8 | 81.2 | 69.4 |
| I449F | 53.3 | 57.1 | 68.6 | 55.7 | 129.8 | 123.4 |
| I449W | 48.8 | 61.7 | 42.3 | 57.0 | 156.2 | 127.9 |
| S443P | 96.4 | 98.2 | 91.6 | 102.0 | 167.5 | 160.5 |
a
Total F1 expression after a 15-min 35S-Met pulse labeling and a 3-h chase.
b
pGEM2X DNA transfected into CV-1 cells, vaccinia vTF7-3 coinfection.
c
pCAGGS DNA transfected into Vero cells.
d
Cell surface expression measured by flow cytometry using the mAb F1A.
e
Reactivity of cell surface–expressed F mutants to the conformation-specific mAb 21-1, as determined by flow cytometry.
f
All values were normalized to SV5 wt F protein.