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. 2003 Oct 27;163(2):215–222. doi: 10.1083/jcb.200305100

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Purification of inner kinetochore proteins. (A) Silver-stained SDS-PAGE gels showing the purification of Mif2p, Cbf1p, the Mtw1 complex, and the CBF3 complex from yeast protein extracts. An asterisk denotes background bands corresponding to the heat shock proteins Ssa1 and Ssa2. (B) Percent sequence coverage obtained from mass spectrometric analysis of each purification shown in A. Brackets show proteins organized into subcomplexes. (C) Schematic diagram showing the associations between the different subcomplexes suggested by their copurification.