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. 2002 Jun 24;157(7):1279–1290. doi: 10.1083/jcb.200203073

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Heparin-binding site in LG4 is required by laminin mediation of basement membrane assembly in laminin γ1-null EBs. (A) Elution of recombinant wild-type and KRK→AAA mutant recombinant LG4–5 from a heparin 5PW column with a NaCl gradient. (B) Immunofluorescence (laminin γ1, type IV collagen) micrographs of γ1-laminin–null EBs treated with either laminin-1 in the presence of a 20-fold molar excess of wild-type or mutated LG4–5. (C) Quantitation of degree of basement membrane formation. (D) Space-filling model of Lm-α1 LG4 KRK sequence (blue) superimposed upon the crystal structure of α2-LG4 determined from coordinates submitted to the Brookhaven protein database.