Table I.
Basement membrane formation and epiblast differentiation in wild-type, dystroglycan-null, γ1-laminin–null, and β1-integrin–null embryoid bodies
| EBs | Treatment | Basement membrane formation | Epiblast differentiation | Number counted | |
|---|---|---|---|---|---|
| %
|
|||||
| Nulls | |||||
| Wild-type | 72 ± 9 | 64 ± 7 | 145 | ||
| DG−/− | 75 ± 4 | 51 ± 6 | 191 | ||
| γ1-Lm−/− | 0 | 0 | 253 | ||
| γ1-Lm−/− | Laminin-1 | 57 ± 9 | 34 ± 5a | 150 | |
| β1-integrin−/− | 0 | 0 | 173 | ||
| β1-integrin−/− | Laminin-1 | 46 ± 2 | 18 ± 2a | 174 | |
| Isoforms | |||||
| γ1-Lm−/− | Laminin-1 (α1β1γ1) | 59 | 36 | 150 | |
| γ1-Lm−/− | Laminin-2/4 (α2β1γ1/α2β2γ1) | 37 | 9 | 35 | |
| γ1-Lm−/− | Laminin-4 (α2β2γ1) | 40 | 20 | 40 | |
| γ1-Lm−/− | Laminin-5 (α3Aβ3γ2) | 0 | 0 | 44 | |
| γ1-Lm−/− | Laminin-8 (α4β1γ1) | 0 | 0 | 42 | |
| Modified laminin-1 and fragments | |||||
| γ1-Lm−/− | AEBSF–laminin-1 | 0 | 0 | 39 | |
| γ1-Lm−/− | Laminin-1 + E1' | 0 | 0 | 39 | |
| γ1-Lm−/− | Laminin-1 + AEBSF-E1' | 53 | 11 | 66 | |
| γ1-Lm−/− | Laminin-1 + E4 | 2 | 0 | 43 | |
| γ1-Lm−/− | Laminin-1 + E3 | 0 | 0 | 58 | |
| γ1-Lm−/− | Laminin-1 + E8 | 39 | 16 | 44 | |
EB, embryoid body.
a
P < 0.001 versus wild type. Data were obtained from four to six separate experiments for each group and expressed as mean ± SE.