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. 2002 Jun 24;157(7):1233–1245. doi: 10.1083/jcb.200112126

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Copyright © 2002, The Rockefeller University Press

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Figure 8. — Localization of phosphorylated ERM proteins and phosphoinositides at the anchoring structure. (A) 4M7 cells adhered to activated HUVEC were fixed, permeabilized and stained with the mAb 297S. Representative horizontal section of a confocal micrograph (a), and a three-dimensional reconstruction of a series of horizontal sections (b) are shown. Bar, 5 μm. (B) HUVEC cells were transfected with PLCs-PH- and GRP1-PH-GFP and then, 4M7 cells were allowed to adhere. Thereafter, cells were fixed, permeabilized and stained with the anti-VCAM-1 mAb P8B1 (a and d). Green signal corresponds to GFP fusion proteins (b and e). Panels c and f show three-dimensional reconstructions of horizontal sections corresponding to the green signal. Bars, 5 μm.