Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Jan 7;156(1):41–52. doi: 10.1083/jcb.200105133

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Characterization of antibodies to ZBP2. Antibodies against ZBP2 were made by injecting SDS-PAGE gel purified ZBP2 into rats. (A) Western blotting to ZBP2. (Lane 1) Crude brain extract. (Lane 2) Crude fibroblast extract. (Lane 3) 1.0 M KCl affinity- purified protein fraction of brain extract. The arrow indicates ZBP2. (B) Antiserum against ZBP2 or preimmune serum was incubated with brain extract for 2 h followed by incubation with agarose-protein G beads for 2 h at 4°C. The supernatants, after centrifugation at 1,500 rpm for 10 min, were analyzed by motility shift assay for their ability to form an RNA–protein complex. (Lane 1) Control brain extract. (Lane 2) Brain extract incubated with rat preimmune serum. (Lane 3) Brain extract incubated with anti-ZBP2 serum. Arrows denote the RNA–protein complexes identified in Fig. 1 A. (C) The same samples as in B were used for Western blotting. (Lane 1) Control brain extract. (Lane 2) Brain extract incubated with rat preimmune serum. (Lane 3) brain extract immunodepleted using anti-ZBP2 serum. Arrow indicates ZBP2.