Table II. Reduced aPKC suppresses Lgl cell polarity and cell overgrowth phenotypes.
| Genotypesa | WT | lgl/lgl | lgl aPKC/lgl aPKC or lgl aPKC/lgl+ |
|---|---|---|---|
| NB basal Miranda (E10)b | 94% | 33% | 72% |
| NB basal Miranda (E15)b | 94% | 7% | 24% |
| Large NB size (E15)b , c | 100% | 88% | 83% |
| NB number (L3)d | 92 ± 6 | 137 ± 10 | 107 ± 4 |
| Brain size (L3)e | 82 ± 8 | 136 ± 12 | 114 ± 11 |
| Imaginal disc size (L3) | Normal | Enlarged | Normal |
| Imaginal disc monolayer (L2/3) | Yes | Never | Yes |
a
The wild-type (WT) stock were y w (rows 1–3), lgl334 aPKC k06403/++ (rows 4 and 7), and lgl 334/+ (rows 5 and 6). The lgl/lgl mutants were lgl4/lgl4 (rows 1–3), lgl4/lgl334 (row 5), and lgl334/lgl334 (rows 4, 6, and 7). The lgl aPKC double mutants were lgl4 aPKCk06403/lgl4 aPKC k06403 (rows 1–3), lgl334 aPKCk06403/lgl 4+ (row 5), and lgl334 aPKCk06403/lgl334+ (rows 4, 6, and 7).
b
Number of neuroblasts (NB) scored is >41 for all genotypes.
c
NB/GMC cell size ratio of >0.8 at telophase (Albertson and Doe, 2003).
d
Neuroblast number per hemisphere; n = 10 for each genotype.
e
Brain size in volume (mm3 × 10−4) per hemisphere; n = 10 for each genotype.