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. 2003 Dec 8;163(5):1045–1055. doi: 10.1083/jcb.200309093

Figure 2.

Figure 2.

Microvillar PAB length is positively correlated with espin level. CL4 cells were transfected with GFP-espin cDNA using a series of CMV promoter deletion constructs. Microvillar PAB length was measured by confocal microscopy (n = 187–555 microvilli, 12–28 cells), and replicate dishes were dissolved in SDS gel sample buffer to measure relative espin level by Western blotting (100% = full strength CMV promoter). (A) Frequency plots of microvillar PAB length for control cells and for cells transfected with two of the espin constructs. (B) Espin concentration dependence of microvillar PAB lengthening (inset, Western blot from duplicate dishes). Error bars, SEM. All groups were significant compared with control: one-way ANOVA (F(4,1615) = 1576, P < 0.0001); Dunnett's test (P < 0.01).