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. 2003 Dec 8;163(5):1045–1055. doi: 10.1083/jcb.200309093

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — FRAP and rhodamine-actin decoration reveal actin incorporation at the microvillar tip and actin treadmilling in the long microvilli of espin-expressing CL4 cells. (A–C) Examples of GFP–β-actin FRAP in microvilli of CL4 cells expressing espin (A), the ΔP₈ΔWH2 espin construct (B), or no espin (C). Recovery times are shown. Arrowhead in C represents a microvillus as it enters field and obscures recovery. Bar, 1 μm. (D–G) Rhodamine-actin labeling in the microvilli of detergent-permeabilized CL4 cells (D and E) 4 h or (F and G) 24 h after transfection with the ΔP₈ΔWH2 espin construct (E and G, with fluorescein-phalloidin counterstain). Bar, 2 μm.