Figure 3.

THL inhibits the neurite outgrowth response stimulated by FGF2. Rat cerebellar neurons were isolated at postnatal day 2 and cultured over monolayers of 3T3 cells in control media or media supplemented with 5 ng/ml FGF2, 5 ng/ml BDNF, or 0.2 μM WIN55,2122-2 as indicated. The experiments were done in the absence (white bars) and presence (black bars) of 10 μM THL. After 18 h, the cultures were fixed and stained for GAP-43, and the mean length of the longest neurite was determined from ∼140 neurons under each culture condition. For the control and FGF2 data, results were pooled from three independent experiments. For the BDNF and CB1 agonist data, the results were pooled from two independent experiments. Bars show SEM. Dose–response experiments (not depicted) indicated an IC₅₀ = 2 μM for the inhibition by THL of FGF2 response.