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. 2003 Nov 24;163(4):767–775. doi: 10.1083/jcb.200308075

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Reversal of eIF2α phosphorylation in the absence of GADD34. (A) Immunoblots of eIF2α phosphorylated on serine 51, detected with an epitope-specific primary antiserum, eIF2α (P), total eIF2α(T), PERK (which detects both the unphosphorylated, inactive form of the kinase PERK° and activated, phosphorylated form PERK(P)), and GADD34 on lysates prepared from mouse embryonic fibroblasts with the indicated GADD34 genotypes. The cells were treated for 30 min with 1 mM dithiothreitol (DTT) and placed in DTT-free media for the indicated period of time (wash). Cells were also treated for 4 h with the ER stress-inducing drug thapsigargin (400 nM) serving as positive control for GADD34 induction. (B) Similar experiment to A performed in mouse embryonic stem cells.

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