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. 2003 Nov 24;163(4):723–728. doi: 10.1083/jcb.200305112

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — Requirement of Notch signaling for the effects of TGF-β on Hes-1 expression. (A) Real-time PCR analysis of Hes-1 mRNA expression in C2C12 mouse myoblasts expressing a dominant-negative CSL construct (DN-CSL) after treatment with TGF-β. Cells expressing DN-CSL (cotransfected with a GFP construct) were selected by FACS^® analysis and treated with TGF-β for 60 min before RNA extraction. Results are presented as the mean ± SD of triplicate determinations. (B) Activation of the Hes-1 promoter in C2C12 myoblasts by NICD and TGF-β. A 500-bp construct of the Hes-1 promoter coupled to a luciferase reporter was introduced into C2C12 cells along with NICD (from mouse Notch1) as indicated. Treatment with TGF-β resulted in potentiation of NICD activity in a dose-dependent manner. Results are presented as the mean ± SD of triplicate determinations.