Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Nov 24;163(4):777–787. doi: 10.1083/jcb.200304112

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Blue native gel electrophoresis of mitochondrial complexes. (A) Coomassie brilliant blue staining of mitoplast preparations. (B) Complex I in situ activity staining by nitro blue tetrazolium reduction assay. (C) Rescue of complex I in situ activity in HSP cells after pSPG7 transfection. (D and E) Western blot analysis of complex I revealed by anti–39-kD antibody. Immunoblotting with HSP60 antibody was used to verify equal loading. Activity and protein amount of complex I were quantified by densitometric analysis. *, P < 0.05; **, P < 0.001. Bars represent ±SEM.