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. 2003 Nov 24;163(4):789–799. doi: 10.1083/jcb.200307130

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — XIAP is necessary for the postcytochrome c regulation of caspase activation in sympathetic neurons. (a) NGF-maintained sympathetic neurons from XIAP-deficient (−/−) or wild-type (+/+) littermate mice were microinjected with either bovine or yeast cytochrome c. Survival of the injected neurons was assessed at the indicated times. Data shown are mean ± SEM of three independent experiments. (b) Phase contrast micrographs of wild-type (XIAP+/+) and XIAP-deficient (XIAP−/−) neurons are shown. Neurons that were injected with bovine cytochrome c (and rhodamine dextran) were identified by fluorescence micrographs for the same field and are marked with arrowheads. Bar, 10 μm. (c) Reintroduction of XIAP into XIAP-deficient neurons restores resistance to cytosolic microinjection of cytochrome c. NGF-maintained, XIAP-deficient sympathetic neurons were coinjected with plasmids expressing either XIAP or vector alone and EGFP. After 24 h to allow for expression, the injected neurons were reinjected with cytochrome c and survival of the cytochrome c–injected cells was determined 12 h after the injections. Data are mean ± SEM of three independent experiments.