Figure 3.

Enrichment for myeloid and erythroid CFCs in the lacZ-positive bone marrow fractions of SCL^lacZ/w mice. (A) Frequency of day 7 myeloid CFC in agar cultures of unsorted and sorted bone marrow fractions from SCL^lacZ/w and SCL^w/w mice cultured in IL-3 or SCF. Values represent the mean ± SD from five experiments for SCL^lacZ/w and the mean from two experiments for SCL^w/w bone marrow cells. The percentage of bone marrow cells that were sorted into each fraction is indicated. (B) Frequency of day 2 CFU-E, day 7 BFU-E, and myeloid (GM) CFC in methylcellulose cultures of unsorted and sorted bone marrow fractions from SCL^lacZ/w mice cultured in Epo (for day 2 CFU-E) or IL-3/Epo (for day 7 BFU-E and GM CFC). Values represent the mean ± SD from three experiments. The percentage of bone marrow cells that were sorted into each fraction is indicated.