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abl M mutants are cold sensitive. Embryos labeled to visualize F-actin were ranked by phenotypic severity. Class I: virtually wild-type with no multinucleate cells. Class II: isolated multinucleate cells. Class III: many multinucleate cells, resulting in patches of actin loss. Class IV: severe actin disruption. Percentage of progeny in each class at 25°C vs. 18°C are indicated. These phenotypic classes are also used in Table I. Bar, 10 μm.
