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. 2003 Dec 22;163(6):1351–1362. doi: 10.1083/jcb.200306067

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — Cadherin–catenin complexes. (A) Immunocytochemistry. Cells were stained with different antibodies indicated at the top of each panel and cell lines are designated at the left side of each panel. Phalloidin-Texas red was used to stain for actin. (B) E-cadherin immunoprecipitation. Cells were extracted in 1% Triton X-100 buffer and equal amounts of cell extract from each cell lines were immunoprecipitated and blotted. Cell lines and antibodies for immunoprecipitation and immunoblot are shown at the bottom and top of each panel, respectively. (C) Inhibition of CD151 expression with shRNA dissociated α-actinin from the cadherin–catenin complex. (D) Tyrosine phosphorylation of β-catenin. Cells were treated with 1 mM sodium orthovanadate before extraction. All buffers used in this experiment were supplemented with 1 mM sodium orthovanadate. The top panel is the phosphotyrosine blot, and the bottom panel is the reblot with the same antibody used for immunoprecipitation. (E) A model summarizing the results of this figure.

Figure 3. — Cadherin–catenin complexes. (A) Immunocytochemistry. Cells were stained with different antibodies indicated at the top of each panel and cell lines are designated at the left side of each panel. Phalloidin-Texas red was used to stain for actin. (B) E-cadherin immunoprecipitation. Cells were extracted in 1% Triton X-100 buffer and equal amounts of cell extract from each cell lines were immunoprecipitated and blotted. Cell lines and antibodies for immunoprecipitation and immunoblot are shown at the bottom and top of each panel, respectively. (C) Inhibition of CD151 expression with shRNA dissociated α-actinin from the cadherin–catenin complex. (D) Tyrosine phosphorylation of β-catenin. Cells were treated with 1 mM sodium orthovanadate before extraction. All buffers used in this experiment were supplemented with 1 mM sodium orthovanadate. The top panel is the phosphotyrosine blot, and the bottom panel is the reblot with the same antibody used for immunoprecipitation. (E) A model summarizing the results of this figure.