Figure 6.

Correlation of a signal's TRAP dependence and post-targeting gating function. The eight signal sequences from Fig. 5 are plotted on a graph that shows their relative dependence on TRAP for translocation on the x axis and their post-targeting gating activity on the y axis. Each signal sequence's gating activity was taken from data reported in previously published work (Kim et al., 2002). In this study, gating was defined as a signal's ability to initiate substrate translocation at the translocon and was measured based on an assay using the topology of PrP as a reporter of the efficiency of NH₂-terminal translocation (Kim et al., 2002).