Figure 8.
Modulation of TRAP dependence by changing signal–translocon interactions. (A) Sequences of the wild-type, N7 mutant, and N9 mutant signal sequences of PrP. The mutated residue is indicated in bold. (B) The N7 and N9 signal sequences fused to Prl were assayed for their dependence on TRAP for translocation, as in Fig. 3 B. Control reactions containing RMs or lacking membranes are also shown for comparison.