Figure 2.

Muscle differentiation of hSM-MSCs recapitulates embryonic myogenesis. (A) hSM-MSCs were injected into regenerating (CTX-treated) TA muscles of nude mice. Mice were killed at different time points as indicated. The dissected muscles were subjected to SQ–RT-PCR analysis. TA muscles containing human cells were equalized for the expression of human β-actin. TA muscles with no human cells served as controls for species specificity of the primers for human cDNAs. For each time point, a PBS-injected regenerating (CTX-treated) mouse TA muscle was included for a negative control. m-TA, uninjected mouse TA muscle; m-CTX-TA, mouse CTX-treated TA muscle. (B) Number of human CE per TA muscle over time as determined by Q–RT-PCR (see Results for details).