Table III. Mitotic stability upon depletion of Chl4p.
| Relevant genotype | Colonies (%)a | No. of colonies scoredb | n b | % Stabilityc (+URA) | ||
|---|---|---|---|---|---|---|
| Red | Red + white sectored | White | ||||
| WT | 16.0 | 67.0 | 17.0 | 980 | 5 | 89 ± 2.0 |
| chl4Δ | <0.5 | 19.0 | 81.0 | 1,397 | 5 | 10 ± 2.0 |
| GAL–UBI–CHL4 | ||||||
| Galactose | 2.0 | 75.0 | 23.0 | 415 | 3 | 80 ± 3.0 |
| Glucose | <0.5 | 13.0 | 86.0 | 2,019 | 8 | 9 ± 3.0 |
| Gal→Glu | 1.0 | 75.0 | 24.0 | 3,287 | 10 | 59 ± 14.0 |
pDLB2064 was transformed into the GAL1–UBI–CHL4 strain on glucose and galactose and selected on either Glu-uracil or Gal-uracil. Single colonies were grown on YPD/YPG overnight and plated on YPD/YPG plates. For the gal→glu transitions, single colonies from galactose transformants were grown on gal-uracil media overnight at 30°C. Cells were washed and shifted to YPD media for 8–10 h and plated onto YPD plates.
Colonies were scored as being red, white, or colonies with red and white sectors after 3 d and expressed as a percent of the total number of colonies scored.
Number of colonies scored is the sum of the number of red, white, or red and white–sectored colonies for each transformant analyzed. n, number of independent transformants assayed.
Expressed as colonies that were URA/colonies on YPD or YPG. For % stability of gal→glu, cells grown overnight in galactose were switched to YPD media for 8–10 h before plating on YPD.