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. 2003 Mar 17;160(6):857–874. doi: 10.1083/jcb.200209097

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Effects of CLA4t overexpression on benomyl sensitivity and cell growth. (A) Schematic representation of wild-type Cla4 and its truncated Cla4t variant. PH, pleckstrin homology domain; PAK, p21-activated kinase family domain. (B and C) Serial dilutions of wild-type (W303), bub2Δ (ySP1071), mad2Δ (ySP1070), and bfa1Δ (ySP1243) strains carrying the indicated 2μ plasmids were spotted on YEPD plates with or without benomyl and incubated for two days at 25°C. (D) Serial dilutions of wild-type (W303) and isogenic strains carrying the indicated numbers of integrated GAL-CLAt fusions were spotted on YEPD (−Gal, GAL1 promoter off) and YEPRG (+Gal, GAL1 promoter on) plates and incubated for two days at 30°C.