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. 2003 Mar 17;160(6):857–874. doi: 10.1083/jcb.200209097

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — Expression of GAL-CLA4t delays degradation of Cdc20/APC substrates with a mechanism partially dependent on Swe1. Wild-type (ySP1969), 1X GAL-CLA4t (ySP3073), swe1Δ 1X GAL-CLA4t (ySP3098), and CDC28 ^Y19F 1X GAL-CLA4t (ySP3074) cells expressing myc-tagged securin (Pds1myc18) were grown in YEPR, arrested in G1 with α-factor, and released in YEPRG (t = 0). Samples were collected at the indicated times for Western blot analysis (A) to score nuclear division (B) and for FACS^® analysis of DNA contents (not depicted). 10 μg/ml α-factor was added back to the cultures at t = 110'.