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. 2003 Mar 17;160(6):857–874. doi: 10.1083/jcb.200209097

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Swe1 is required for Cla4t-dependent stabilization of Clb2 in nocodazole-treated spindle checkpoint mutants. (A) 1X GAL-CLA4t (ySP2622), mad2Δ (ySP1070), mad2Δ 1X GAL-CLA4t (ySP2752), mad2Δ swe1Δ 1X GAL-CLA4t (ySP3145), and mad2Δ CDC28 ^Y19F 1X GAL-CLA4t (ySP3148) cells (A) or 1X GAL-CLA4t (2622), bub2Δ (ySP3138), bub2Δ 1X GAL-CLA4t (ySP2626), and bubΔ swe1Δ 1X GAL-CLA4t (ySP2726) cells (B) were grown in YEPR, arrested in G1 with α-factor, and released in YEPRG (t = 0). Samples were collected at the indicated times for Western blot analysis of Clb2 and Sic1 and for FACS^® analysis of the DNA contents (not depicted). 10 μg/ml α-factor was added back to the cultures at t = 120'. Swi6 was used as loading control. Cyc, cycling cells.

Figure 5. — Swe1 is required for Cla4t-dependent stabilization of Clb2 in nocodazole-treated spindle checkpoint mutants. (A) 1X GAL-CLA4t (ySP2622), mad2Δ (ySP1070), mad2Δ 1X GAL-CLA4t (ySP2752), mad2Δ swe1Δ 1X GAL-CLA4t (ySP3145), and mad2Δ CDC28 ^Y19F 1X GAL-CLA4t (ySP3148) cells (A) or 1X GAL-CLA4t (2622), bub2Δ (ySP3138), bub2Δ 1X GAL-CLA4t (ySP2626), and bubΔ swe1Δ 1X GAL-CLA4t (ySP2726) cells (B) were grown in YEPR, arrested in G1 with α-factor, and released in YEPRG (t = 0). Samples were collected at the indicated times for Western blot analysis of Clb2 and Sic1 and for FACS^® analysis of the DNA contents (not depicted). 10 μg/ml α-factor was added back to the cultures at t = 120'. Swi6 was used as loading control. Cyc, cycling cells.