Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Mar 17;160(6):857–874. doi: 10.1083/jcb.200209097

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — GAL-CLA4t expression delays MEN activation and prevents proper localization of Lte1 at the bud cortex. (A) Serial dilutions of 1X GAL-CLA4t (ySP2622), cdc14–3 (ySP284), cdc14–3 1X GAL-CLA4t (ySP3028), cdc14–3 swe1Δ 1X GAL-CLA4t (ySP3047), cdc15–2 (ySP51), cdc15–2 1X GAL-CLA4t (ySP3022), and cdc15–2 swe1Δ 1X GAL-CLA4t (ySP3023) strains were spotted on either YEPD (−Gal) or YEPRG (+Gal) plates and incubated for 2 d at 26°C. (B) Wild-type, cdc15–2, and cdc14–3 cells either lacking (W303, ySP51, and ySP284) or carrying (ySP2622, ySP3022, and ySP3028) one copy of GAL-CLA4t were grown in YEPR, arrested in G1 with α-factor, and released in YEPRG (t = 0). Samples were collected every 30' for FACS^® analysis of the DNA contents (not depicted) and to score nuclear division. (C) Wild-type (ySP3222), 1X GAL-CLA4t (ySP3220, not depicted), and 4X GAL-CLA4t (ySP3221) cells were treated as in B. Samples were collected every 15' for 150' for FACS^® analysis of the DNA contents (not depicted) and to score localization of GFP-Lte1 and actin by rhodamine-phalloidin. Photographs were taken 75' after release. Bar, 5 μm.