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. 2003 Mar 17;160(6):857–874. doi: 10.1083/jcb.200209097

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Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 7. — Bub2 and Swe1 act synergistically in regulating mitotic exit. 4X GAL-CLA4t (ySP2625), bub2Δ 4X GAL-CLA4t (ySP2630), swe1Δ 4X GAL-CLA4t (ySP2711), and bub2Δ swe1Δ 4X GAL-CLA4t (ySP2728) cells were grown in YEPR, arrested in G1 with α-factor, and released in YEPRG medium (t = 0). Samples were collected at the indicated times for FACS^® analysis of DNA contents (A) and to follow kinetics of spindle assembly/disassembly by α-tubulin immunostaining (B). Photographs were taken 3 h after release. Bar, 5 μm.