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. 2003 Aug 18;162(4):575–585. doi: 10.1083/jcb.200303143

Figure 2.

Figure 2.

Stimulation of the GTPase activity of the SRP–SR complex by RNC preparations. (A) GTPase assays were conducted in a physiological ionic strength buffer (buffer C, 150 mM K+) and contained pPL86 RNCs (all symbols) and were supplemented with 50 nM SR proteoliposomes (triangles and squares) and 50 nM SRP (squares). (B) RNCs and SRP–RNCs were assembled by translation of pPL86 mRNA in the absence or presence of SRP. The GTPase assays in buffer C (RNCs and SRP–RNCs) were supplemented with 25 nM SR proteoliposomes and 50 nM SRP as indicated. The assay designated LS lacked RNCs and was conducted in a low ionic strength buffer (buffer E, 50 mM K+). (C and D) GTPase assays in buffer C contained pG64 RNCs (C), ffluc77 RNCs (C), pPL86 RNCs (D), or mock-assembled RNCs (D) and were supplemented with 50 nM SR proteoliposomes and 50 nM SRP as indicated. (B–D) The GTPase activity was calculated using 0-, 3-, 6-, and 10-min time points.