Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Aug 18;162(4):551–563. doi: 10.1083/jcb.200303167

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — HeLa cells depleted of CENP-E recruit reduced amounts of BubR1 to their kinetochores and have many misaligned chromosomes. (A) HeLa cells depleted of CENP-E (green) by RNAi (bottom) recruit less BubR1 (red) to their kinetochores than HeLa cells treated with control RNAi (top) after a 30-min treatment with 200 ng/ml colcemid. DNA, blue. (B) Quantitation of the normalized integrated intensity of BubR1 signals at kinetochores in HeLa cells (top) and primary MEFs (bottom). Control kinetochores (blue bars) recruit more BubR1 than CENP-E–depleted kinetochores (red bars). ≥800 kinetochores from ≥10 cells were quantitated for each bar. **, P < 0.001. Error bars represent standard error. (C) Histogram showing the number of misaligned chromosomes per pseudo metaphase cell in CENP-E^Δ/Δ MEFs (red bars) and in HeLa cells depleted of CENP-E by RNAi (green bars).