Table I. Diminished kinetochore recruitment of BubR1 in the absence of CENP-E.
| Prometaphase | Metaphase | Pseudo meta aligned | Pseudo meta polar | |||||
|---|---|---|---|---|---|---|---|---|
| CENP-E genotype | +/+ | Δ/Δ | +/+ | Δ/Δ | +/+ | Δ/Δ | +/+ | Δ/Δ |
| BubR1 | 1.00 (0.13) | 0.52 (0.06) | 0.35 (0.05) | 0.13 (0.02) | NA | 0.03 (0.01) | NA | 0.52 (0.08) |
| Bub1 | 1.00 (0.16) | 0.95 (0.09) | 0.41 (0.04) | 0.36 (0.04) | NA | 0.33 (0.05) | NA | 2.00 (0.14) |
| Mad1 | 1.00 (0.18) | 0.53 (0.13) | 0.37 (0.08) | 0.17 (0.10) | NA | 0.03 (0.01) | NA | 1.88 (0.31) |
| Mad2 | 1.00 (0.10) | 0.48 (0.14) | NA | NA | NA | NA | NA | 2.62 (0.40) |
Values represent normalized, integrated intensities of kinetochore-associated BubR1, Bub1, Mad1, and Mad2, as detailed in Materials and methods. Standard errors are in parentheses.