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. 2003 Aug 18;162(4):683–692. doi: 10.1083/jcb.200211056

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — The molar ratio between cPLA₂ and NADPH oxidase in the membranes of stimulated cells. Samples of recombinant cPLA₂ or p47^phox and membranes (bottom inset) of neutrophils stimulated with 1 mg/ml OZ, 5 × 10⁻⁷ M fMLP for 1 min or 50 ng/ml PMA for 3 min (top inset) were analyzed by SDS-PAGE electrophoresis. 2 × 10⁶ cell membrane equivalents were applied per lane. The Western blots were analyzed using quantitative densitometry, and the relative optical density of each band was plotted against the amount of protein added per lane. The correlation coefficient for the cPLA₂ standard curve is 0.99 and for the p47^phox standard curve is 0.92. The quantities of cPLA₂ and p47^phox in membranes of stimulated cells were determined from the standard curves. The results are from one representative experiment out of three presenting identical results.