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. 2003 Aug 18;162(4):683–692. doi: 10.1083/jcb.200211056

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Binding between cPLA₂ and cytosolic oxidase components. Affinity-binding assay using GST-p67^phox or GST-p47^phox was performed as described in Materials and methods. GST-p67^phox binds p47^phox in lysates of resting and stimulated neutrophils (with 50 ng/ml PMA) and binds cPLA₂ only in lysates of stimulated cells, whereas GST alone was not effective. Similarly, GST-p47^phox binds p67^phox in lysates of resting and stimulated cells and binds cPLA₂ only in lysates of stimulated cells.