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. 2003 Aug 18;162(4):587–597. doi: 10.1083/jcb.200305149

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — ER stress induces enhanced Bbc3/PUMA mRNA and protein levels. (A) Expression of bbc3/PUMA mRNA during tunicamycin-induced ER stress in SH-SY5Y neuroblastoma cells determined by Northern blot analysis. (B) Kinetics of Bbc3/PUMA protein expression and caspase-3 activation during tunicamycin- induced cell death in SH-SY5Y neuroblastoma cells. Whole-cell extracts of cells treated for the indicated times with 3 μM tunicamycin or vehicle (DMSO) were analyzed by Western blot analysis. Membranes were probed for either Bbc3/PUMA expression levels or caspase-3 activation. Arrowheads indicate active caspase-3 subunits (p20, p17, and p14). α-Tubulin served as a loading control. Similar results were obtained in two separate experiments. (C) Bim and Bid protein expression during tunicamycin-induced cell death in SH-SY5Y neuroblastoma cells. Cells were treated as described above.