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. 2003 Aug 18;162(4):587–597. doi: 10.1083/jcb.200305149

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — ER stress is able to induce bbc3/PUMA in a p53- independent manner. (A) Tunicamycin treatment does not lead to an accumulation of p53 and p21 in SH-SY5Y cells. p53 and p21 protein levels after exposure to tunicamycin (TU; 3 μM, 24 h), etoposide (ETO; 50 μM, 24 h), or vehicle (controls; DMSO, 24 h) were evaluated by Western blotting. (B and C) Kinetics of ER stress–induced bbc3/PUMA mRNA and protein levels after 3 μM tunicamycin treatment in p53-deficient human SAOS-2 osteosarcoma cells determined by Northern and Western blot analyses.