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. 2003 Aug 18;162(4):565–574. doi: 10.1083/jcb.200305013

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — Localization of DNA probes A, B, and C and Su(Hw) protein on polytene chromosomes. BAC clones A, B, or C were used as DNA probes for FISH of polytene chromosomes from salivary glands of Drosophila third instar larvae from wild-type or ct ⁶ mutant strains. The chromosomes were simultaneously stained with antibodies against the Su(Hw) protein. For all images, the DNA is visualized by DAPI staining (blue), and the locations of endogenous Su(Hw) binding sites at 7B2 and 7B8, indicated by Su(Hw) staining (red), are labeled with arrows. (A) Schematic representation of probes A, B, and C at the ct locus. The ct ⁶ mutant contains a copy of the gypsy retrotransposon located in the region recognized by probe B. The location of the gypsy retrotransposon and overlap of probes A, B, and C are drawn to scale. (B–D) Immunolocalization of Su(Hw) (red) and FISH signal of probe A in polytene chromosomes from wild-type larvae. (E–G) Immunolocalization of Su(Hw) (red) and FISH signal of probe B (green) in polytene chromosomes from wild-type larvae. (H–J) Immunolocalization of Su(Hw) (red) and FISH signal of probe C (green) in polytene chromosomes from wild-type larvae. (K–M) Immunolocalization of Su(Hw) (red) and FISH signal of probe B in polytene chromosomes from ct ⁶ larvae.