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. 2003 Aug 18;162(4):693–701. doi: 10.1083/jcb.200302051

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Cells overexpressing PIP5KIα or β have more AP-2 on membranes. Cytosol (S100) and crude membrane fractions (P100; see Materials and methods) from cells overexpressing PIP5KI enzymes or β-galactosidase (C) were resolved by 10% SDS-PAGE, transferred to nitrocellulose membranes, and blotted with antibody specific for α-adaptin. An example of such a blot is shown. Averages from three independent experiments are graphed with SD.