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. 2003 Aug 18;162(4):693–701. doi: 10.1083/jcb.200302051

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Reduced expression of PIP5KIβ (but not α or γ) reduces the rate of transferrin internalization. (A) The internalization of transferrin was measured in cells treated with siRNA specific for one of the three PIP5KI isoforms as described in Materials and methods. (B) Cytosol (S100) and crude membrane fractions (P100; see Materials and methods) from cells with reduced levels of PIP5K were resolved by SDS-PAGE and were Western blotted with antibody specific for α-adaptin. A representative blot showing duplicate samples treated with siRNA targeting PIP5KIβ and densitometric analysis of four experiments are shown. Error bars indicate the SD.

Figure 8. — Reduced expression of PIP5KIβ (but not α or γ) reduces the rate of transferrin internalization. (A) The internalization of transferrin was measured in cells treated with siRNA specific for one of the three PIP5KI isoforms as described in Materials and methods. (B) Cytosol (S100) and crude membrane fractions (P100; see Materials and methods) from cells with reduced levels of PIP5K were resolved by SDS-PAGE and were Western blotted with antibody specific for α-adaptin. A representative blot showing duplicate samples treated with siRNA targeting PIP5KIβ and densitometric analysis of four experiments are shown. Error bars indicate the SD.