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. 2002 Aug 5;158(3):577–590. doi: 10.1083/jcb.200202083

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Synaptic localization of CAST. (A) Gross distribution of CAST in mouse brain. (B) CA3 region of mouse hippocampus. The sections were doubly stained using the anti–CAST-2 and anti–synaptotagmin I Abs. (a) Low magnification. (b) High magnification. SR, stratum radiatum; SL, stratum lucidum; SP, stratum pyramidale; SO, stratum oriens. Bars: (a) 100 μm; (b) 30 μm. (C) Primary cultured rat hippocampal neurons. Neurons at 21 d of culture were doubly stained using the anti–CAST-2 and antibassoon Abs or anti–PSD-95/SAP90 Ab. Arrows indicate the colocalized signals. Bars, 10 μm. These results are representative of three independent experiments.