Table III. Analysis of activated caspase-3 in cells expressing E4orf4-GFP proteins.

	C-33A			293T
	Caspase 3	Apoptosis		Caspase 3	Apoptosis
	+	% cells			+	% cells
	% cells	−zVAD	+zVAD	% cells	−zBocD	+BocD
GFP	7b	7	6	0.2c	2	3
E4orf4-GFP	24b	33	38	0.8c	52	48
GFP-CAAX	7b	10	8	0.4c	5	6
E4orf4-GFP-CAAX	24b	34	32	2.4c	40	41
GFP-NLS	12b	10	9	1.0d	2	3
E4orf4-GFP-NLS	23b	23	20	–	–	–
3YF-GFP-NLS	–	–	–	2.1d	27	34
Bax-GFP	93a	98	51	80.0a	100	21
	n > 350					n > 500

^aCells fixed at 24 h.

^bCells fixed at 36 h.

^cCells fixed at 48 h.

^dCells fixed at 72 h.

Apoptosis was determined from the total number of E4orf4-expressing cells with apoptotic nuclei (including condensed “c” or fragmented “f”, Fig. 4 C) and the percentages of apoptotic cells showing positive staining for active caspase-3 (Caspase-3 [+]) were determined in absence and presence (data not shown) of caspase inhibitors. Data are representative of at least two independent experiments.