Figure 2.

Cre-mediated deletion of c-jun. Sympathetic neurons isolated from c-jun floxed (c-jun ^fl/fl) or wt mice were cultured for 4 d with NGF, then were infected overnight with an adenovirus carrying Cre recombinase (A). The neurons were rinsed and refed with media lacking NGF and supplemented with a neutralizing anti-NGF antibody (−NGF) and the cells were fixed 12 h later. Co-immunostaining with antibodies to Cre recombinase (green) and c-Jun (red) was performed to identify Cre-infected neurons and to detect c-Jun expression. Neurons were also stained with DAPI (blue) to detect apoptotic profiles. Note that c-Jun was not detected in Cre- immunopositive neurons from c-jun ^fl/fl mice. (B) Genomic DNA was isolated from sympathetic neurons, uninfected or 48 h after infection with adenovirus expressing Cre recombinase or GFP, and PCR was performed to detect the presence of c-jun and, as control, the p65 subunit of NF-κB.