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. 2002 Aug 5;158(3):453–461. doi: 10.1083/jcb.200112129

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — BDNF induces apoptosis through the p75 receptor. Sympathetic neurons isolated from SCG of postnatal day 1 mice were cultured 3–5 d with 20 ng/ml NGF. The neurons were rinsed and refed with media lacking NGF and containing a neutralizing anti-NGF antibody and 12.5 mM KCl with (+BDNF) or without (−BDNF) 100 ng/ml BDNF, to activate p75. In some experiments, antibodies to the extracellular domain of p75 were included (diluted 1:500). After 48 h, the neurons were fixed and apoptotic cells were identified by their nuclear profile using DAPI staining. The percentage of apoptotic nuclei was determined by counting at least 50 neurons in six independent experiments, and the mean and SEM are shown. Only the neurons treated with BDNF and no antibody were significantly different from the others, based on an ANOVA analysis and a Tukey's multiple comparison test.