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. 2002 May 13;157(4):631–643. doi: 10.1083/jcb.200111081

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — Sec34p interacts with retrograde Golgi SNAREs and with COPI. GST-Sec34p was expressed in a Δsec34 yeast strain. Affinity chromatography on glutathione–Sepharose beads was employed to purify GST-Sec34p and associated proteins from a P100 fraction (Total) that was solubilized in CHN buffer (20 mM Hepes, pH 7.4, 1% CHAPS, 0.15 M NaCl). The beads were eluted with 10 mM glutathione (Eluate). As a control, the GST protein was expressed and purified from the membrane fraction of sec34/Δsec34 strain. The GST-Sec34p– and Sec34-associated proteins were identified by immunoblotting with antibodies to A, SNARE proteins and Sec35p; (B) anti-COPI, Sec21p, or Sec13p. (C) Coomassie blue staining of the GST and GST-Sec34p eluates.

Figure 5. — Sec34p interacts with retrograde Golgi SNAREs and with COPI. GST-Sec34p was expressed in a Δsec34 yeast strain. Affinity chromatography on glutathione–Sepharose beads was employed to purify GST-Sec34p and associated proteins from a P100 fraction (Total) that was solubilized in CHN buffer (20 mM Hepes, pH 7.4, 1% CHAPS, 0.15 M NaCl). The beads were eluted with 10 mM glutathione (Eluate). As a control, the GST protein was expressed and purified from the membrane fraction of sec34/Δsec34 strain. The GST-Sec34p– and Sec34-associated proteins were identified by immunoblotting with antibodies to A, SNARE proteins and Sec35p; (B) anti-COPI, Sec21p, or Sec13p. (C) Coomassie blue staining of the GST and GST-Sec34p eluates.