Table I. Plasmids used in this work.
| Plasmid | Description | Source |
|---|---|---|
| pYGST-TFI1 | P Gal -GST-TFI1 in pEMBLyex4 (2 μ, URA3) | This study |
| pYGST-TFI2 | P Gal -GST-TFI2 in pEMBLyex4 (2 μ, URA3) | This study |
| pYGST-SEC34 | P Gal -GST-SEC34 in pEMBLyex4 (2 μ, URA3) | This study |
| pYGST-TFI3 | P cup1 -GST-TFI3 in pYEX4t-1mod | Invitrogen |
| pNB167 | YPT1, 2 μ, URA3 | S. Ferro-Novicka |
| pWB-Acycα | P CYC1 -SEC22-myc-α, CEN, URA3 | H.D. Schmitt |
| pSV29 | GST-SEC35 in pGEX4T | M.G. Watersb |
| pGST-Sed5 | GST-SED5(1-324) in pGEX2T | M.G. Waters |
| pGAL10-GST-YPT1 | GST-YPT1 in pEMBLyex4 (2 μ, URA) | N. Segevc |
| pSV30 | His 6 -SEC34 in pQE30 | M.G. Waters |
a
Yale University, New Haven, CT.
b
Princeton University, Princeton, NJ.
c
University of Chicago, Chicago, IL.