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. 2002 May 13;157(4):565–570. doi: 10.1083/jcb.200202010

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Affinity precipitation of RhoA in postnatal cerebellar neurons. (A) RhoA activity was increased after the addition of MAG–Fc (25 μg/ml). RhoA activity is indicated by the amount of RBD-bound RhoA normalized to the amount of RhoA in the lysates. Values represent RhoA activity relative to the cells at time 0. Results are means ± SE from three experiments. Asterisks indicate statistical significance (*p < 0.01, Student's t test). (B) NGF rapidly inhibits RhoA activity (∼10 min). (C) Dose response. (D) The activation was lost in the neurons from mice carrying a mutation in the p75^NTR gene.