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. 2002 May 13;157(4):591–602. doi: 10.1083/jcb.200202047

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — During mitosis in tbg-1(RNAi) embryos, centrosomal α-tubulin fluorescence reaches ∼40% of wild-type levels. (A) Panels summarizing movies of wild-type (left) and tbg-1(RNAi) embryos (right), expressing GFP–α-tubulin. Times are with respect to NEBD. See also Videos 3 and 4 located at http://www.jcb.org/cgi/content/full/jcb.200202047/DC1. In wild type, asters are visible at an early stage (− 477 s). The asters grow in size (all panels) and become the poles of the mitotic spindle (+150 s). (−447 s) In tbg-1(RNAi) embryos there are no detectable asters at early time points. (−180 s) Coincident with the onset of mitotic prophase in wild-type (judged by comparison of GFP–α-tubulin and GFP–histone sequences), centrosomal asters form in the tbg-1(RNAi) embryos. At this time, centrosomes (arrow) are often observed to “fly in” to meet the paternal pronucleus. (+189 s) After NEBD, robust mitotic asters form but no spindle assembles. Bar, 10 μm. (B) Kinetic traces of centrosomal α-tubulin fluorescence. Centrosomal fluorescence was quantitated in five wild-type and four tbg-1(RNAi) embryos. Three traces are shown for each.